The only other chamber off the main room in Ay's tomb was made to hold his canopic chest. Above the doorway is a painting showing the four sons of Horus, the guardians of the four canopic jars. It was only at the end of the 18th dynasty that they were depicted with animal heads. On the left are Duamutef, who protected the stomach and Qebehsenuef, who protected the intestines - these are wearing the crown of Upper Egypt. On the right are Imesty, who protected the liver, and Hapi, protecting the lungs - these are wearing the crown of Lower Egypt.

The heavily restored sarcophagus was returned from Cairo a few years ago. At the four corners, the four protective goddesses, Isis, Nephtys, Selkhet and Neith wrap their wings around it. Neith is shown in the photograph on the right.